Composite

Part:BBa_K3581010:Design

Designed by: HUI Wan Hei   Group: iGEM20_HKUST   (2020-10-13)


BBa_K880005 part without hagfish intermediate filament subunits with HlyA signal peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 55
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The hagfish subunit is removed from this part for maximizing protein secretion even if the protein is not hagfish intermediate filaments.


Source

All parts are from iGEM parts registry.

References

1. England, N. (n.d.). TEV Protease | NEB. Retrieved October 13, 2020, from https://international.neb.com/products/p8112-tev-protease#Product%20Information

2. Wikipedia. (2020, October 14). TEV Protease. Wikipedia. Retrieved October 13, 2020, from https://en.wikipedia.org/wiki/TEV_protease

3. Palmer, I., & Wingfield, P. T. (2004). Preparation and extraction of insoluble (inclusion-body) proteins from Escherichia coli. Current protocols in protein science, Chapter 6, Unit–6.3. https://doi.org/10.1002/0471140864.ps0603s38

4. https://parts.igem.org/Part:BBa_K880005

5. https://parts.igem.org/Part:BBa_B0015